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cn (ng cn)  (NextGen Sciences)

 
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    NextGen Sciences cn (ng cn)
    Cn (Ng Cn), supplied by NextGen Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cn (ng cn)/product/NextGen Sciences
    Average 90 stars, based on 1 article reviews
    cn (ng cn) - by Bioz Stars, 2026-05
    90/100 stars

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    ( A ) Chromosomal construction of Escherichia coli HK1160 ( P BAD -dCas9-NG-KmR) and structure of sgRNA plasmid. dCas9-NG protein is expressed by L-arabinose-inducible P BAD promoter. sgRNA is constitutively expressed by P j23119 promoter. ( B ) Regulation of gal operon transcription by dCas9-NG and sgRNA complex. In the absence of L-arabinose, dCas9-NG is not expressed and gal transcription can be initiated by RNA polymerase. In the presence of L-arabinose, dCas9-NG is expressed and dCas9-NG-sgRNA complex recognizes target DNA adjacent to functional PAM sequence. ( C ) Switch on/off of L-arabinose-inducible CRISPRi. In the presence of L-arabinose, Cas9 and dCas9 are expressed to repress the expression of D-galactose metabolizing enzymes (white colonies on MacConkey agar containing D-galactose). In the absence of L-arabinose, cells can utilize D-galactose (red colonies).

    Journal: Journal of Microbiology and Biotechnology

    Article Title: Effective Blocking of Microbial Transcriptional Initiation by dCas9-NG-Mediated CRISPR Interference

    doi: 10.4014/jmb.2008.08058

    Figure Lengend Snippet: ( A ) Chromosomal construction of Escherichia coli HK1160 ( P BAD -dCas9-NG-KmR) and structure of sgRNA plasmid. dCas9-NG protein is expressed by L-arabinose-inducible P BAD promoter. sgRNA is constitutively expressed by P j23119 promoter. ( B ) Regulation of gal operon transcription by dCas9-NG and sgRNA complex. In the absence of L-arabinose, dCas9-NG is not expressed and gal transcription can be initiated by RNA polymerase. In the presence of L-arabinose, dCas9-NG is expressed and dCas9-NG-sgRNA complex recognizes target DNA adjacent to functional PAM sequence. ( C ) Switch on/off of L-arabinose-inducible CRISPRi. In the presence of L-arabinose, Cas9 and dCas9 are expressed to repress the expression of D-galactose metabolizing enzymes (white colonies on MacConkey agar containing D-galactose). In the absence of L-arabinose, cells can utilize D-galactose (red colonies).

    Article Snippet: Second, 3’-part (837 bp) of cas9-NG gene was codon-optimized using Codon Optimization Tool ( https://sg.idtdna.com/pages/tools/codon-optimization-tool ) and chemically synthesized (Integrated DNA Technologies, USA).

    Techniques: Plasmid Preparation, Functional Assay, Sequencing, Expressing